iGEM STOCKHOLM 2016
Genetically engineered spider web for healing chronic wounds
iGEM (International Genetically Engineered Machine) is a competition in Synthetic Biology, where students work together to solve real-world challenges by building genetically engineered biological systems with standard, interchangeable parts. We were part of the Stockholm team of 14 people from diverse backgrounds.
The project aimed to construct a functional dressing for chronic wounds, using spider silk as a scaffold to attach biofilm-degrading enzymes. This includes:
Expressing biofilm-degrading ‘combat proteins’ and quantifying their potency
Assembling combat proteins with a linker and tag needed for purification and attachment to spider silk
Attaching the enzymes to spider silk using Sortase A, a transpeptidase
solating Sortase A from the S. aureus genome and creating a ‘Sortase Package’ including basic parts needed for other iGEM teams to use Sortase in their own projects
Testing the efficacy of the fully assembled wound dressing with attached enzymes on biofilms
We worked with imagining the implication of the project, and with design and communication: websites, presentations, holding workshops discussing synthetic biology etc.
Our team was awarded with a Gold Medal, and was also nominated for “Best Presentation” and “Best Education and Public Engagement”.